Journal: Cell Death Discovery

Article Title: Pregnane X receptor protects against age-related bone loss in males via PI3K/Akt-mediated inhibition of apoptosis

doi: 10.1038/s41420-025-02797-y

Figure Lengend Snippet: A Schematic diagram of experimental design. B ELISA for inflammation factors Tnfα, Il1β, and Il6 in the conditional medium collected from Nr1i2 knockdown primary BMSCs or siNC as control. C ELISA for ROS clearance-related enzyme T-SOD, GSH-PX and ROS damage biomarker MDA in Nr1i2 knockdown primary BMSCs or siNC as control. D Flow cytometry analysis of intracellular ROS level by using the fluorescent dye DCFDA. Western blot for PI3K/Akt pathway- ( E ) and apoptosis- ( F ) related proteins in primary BMSCs of different groups. Data were means ± s.e.m. n = 3 independent repeats for in vitro experiments. ** p < 0.01, *** p < 0.001 by t-test.

Article Snippet: The Il1β, Il6, and Tnfα level in the conditional medium, intracellular level of T-SOD, GSH-PX, MDA, and ROS were determined by using the Mouse IL-1β ELISA Kit (HJ177, Epizyme Biotech, Shanghai, China), Mouse IL-6 ELISA Kit (HJ182, Epizyme Biotech, Shanghai, China), and Mouse TNF-α ELISA Kit (HJ207, Epizyme Biotech, Shanghai, China), Total superoxide dismutase (SOD) assay kit (A001-3-2, Jiancheng, Nanjing, China), Glutathione Peroxidase (GSH-PX) Assay Kit (A005-1-2, Jiancheng, Nanjing, China), Malondialdehyde (MDA) assay kit (A003-1-2, Jiancheng, Nanjing, China), and DCFDA/H2DCFDA-Cellular ROS Assay Kit (ab113851, abcam, Cambridge, UK) according to the manufacturer’s instructions, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Knockdown, Control, Biomarker Discovery, Flow Cytometry, Western Blot, In Vitro

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia

doi: 10.1016/j.rpth.2023.102276

Figure Lengend Snippet: Levels of activated FXI were elevated in a model of hyperlipidemia. PPP was isolated from NHPs on a standard chow diet ( n = 6, lean) or NHPs on a high-fat diet ( n = 8, obese). Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl 2 to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT. Activated coagulation protease species, FXIa–AT and T-AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Mann–Whitney test or an unpaired t -test. Statistical significance is indicated by an asterisk for P ≤ .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; PPP, platelet-poor plasma; PT, prothrombin time; NHP, nonhuman primate.

Article Snippet: Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions.

Techniques: Isolation, Coagulation, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia

doi: 10.1016/j.rpth.2023.102276

Figure Lengend Snippet: FXI inhibition prolonged aPTT clotting times in a model of hyperlipidemia. PPP was isolated from NHPs on a high-fat diet ( n = 5) treated with a FXI function–blocking antibody. Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl 2 to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT (B). Activated coagulation protease species, FXIa–AT and T–AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Friedman test with a Dunn’s post-hoc test or repeated measures one-way ANOVA with a Dunnett’s post-hoc test. Statistical significance is indicated by an asterisk for P < .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; NHP, nonhuman primate; PPP, platelet-poor plasma; PT, prothrombin time.

Article Snippet: Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions.

Techniques: Inhibition, Coagulation, Isolation, Blocking Assay, Enzyme-linked Immunosorbent Assay